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Archive for December, 2009

HELP ! AML, M6(erythremic myelosis or Diguglielmo disease-remission criteria

<AML, M6(erythremic myelosis or Diguglielmo disease-remission ciretria>

Dear Sir  
  I am a clinical pathologist working for Dankook University Hospital.
  I would like to have a good opinion for a bone marrow study case.  
  The patient is a 27 year old woman whose bone marrow study shows
 hypercellular marrow and most of cells composed of erythroid precursor
 cells, predominently pronormoblasts. Followings are detail.  
  M:E ratio is about 0.043:1(1:22.7).  
  Erythroblasts are increased and counted upto 86.2 % of ANC.
  Pronormoblasts predominates(about 38.6 % of ANC).  
  Granulopoisis is markedly decreased(5.6%).  
  Nonerythroid blasts are not increased, and they are
 counted about 0.8% of NEC.  
  Megakarycytes are hardly observed.  
  Dyserythropoietic changes are also observed.  
  In the biopsy section, cellularity is about 95% and marrow space is
 almostly packed with erythroblasts.
 So, We diagnosed it as AML, M6(Erythremic myelosis type).
  After induction chemotherapy, second BM study shows 6% of
 pronormoblast, 1.5:1 of ME ratio, 20 % of cellularity, and focal
  erythoid precusors clusters in biopsy specimen.
 Generally, the focus in the remission criteria of AML is myeloblast
count.
 But this bone marrow is richly occupied by erythriod series not
 associated with a myeloblast component.
 What I want to know is the remission criteria of erythremic myelosis.
 Please let me know if you have a good opinion on my case.  

 Sincerely.
 from JongWan Kim, MD. (wan1…@hitel.kol.co.kr)

.
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help

Hello,

I have a question about a procedure I am trying to perfect and was hoping
that you may be somewhat helpful.  I am using 6 micron sections on + slides
and performing a basic immunohistochemical battery (Vector ABC kit).  My
problem involves a high degree of DAB residue (not background) on the
slides.  I have attempted to alleviate this situation by extending washing
periods, I have also tried the procedure on Lysine-subbed slides and am
having the same the residue appear.  The procedure works great on gel
mounted slides but I am unable to use these slides because of a melanin
bleaching procedure required in addition to the traditional
immunohistochemical battery.  Any help you could provide would be greatly
appreciated.

Sincerely,

David
email dkvar…@rush.edu

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Medical and forensic illustrations of all sorts

This is a multi-part message in MIME format.

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Content-Type: text/plain; charset=ISO-8859-1
Content-Transfer-Encoding: 7bit

Excellent freehand  MEDICAL  and  FORENSIC  ILLUSTRATOR  from Austria.

Hi!

I am a recent immigrant from Austria who would like to establish herself in
this country as Medical Illustrator or Forensic Illustrator.  I do
excellent work which is very accurate and professional.  Have years of
experience in dissecting bodies and 5 years of studies towards M.D. in
Austria.  I will be happy to send samples of my illustrations and my resume
to anyone who is seriously interested in hiring an excellent and
scientifically qualified illustrator either for a position of for
free-lance work.  

Thank you,
Elisabeth

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Elisabeth Frauendorfer, PhD                             draw4…@gte.net
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
http://www.freeyellow.com/members/phoenixresearch/index.html
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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Content-Transfer-Encoding: quoted-printable

<html><head></head><BODY bgcolor=3D"#FFFFFF"><p><font size=3D2 =
color=3D"#000000" face=3D"Arial">Excellent freehand &nbsp;MEDICAL =
&nbsp;and &nbsp;FORENSIC &nbsp;ILLUSTRATOR &nbsp;from =
Austria.<br><br><br>Hi!<br><br>I am a recent immigrant from Austria who =
would like to establish herself in this country as Medical Illustrator =
or Forensic Illustrator. &nbsp;I do excellent work which is very =
accurate and professional. &nbsp;Have years of experience in dissecting =
bodies and 5 years of studies towards M.D. in Austria. &nbsp;I will be =
happy to send samples of my illustrations and my resume to anyone who is =
seriously interested in hiring an excellent and scientifically qualified =
illustrator either for a position of for free-lance work. =
&nbsp;<br><br>Thank =
you,<br>Elisabeth<br><br><br>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~=
~~~~~~~~<br>Elisabeth Frauendorfer, PhD =
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&=
nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&n=
bsp;&nbsp;&nbsp;&nbsp;<font =
color=3D"#0000FF"><u>draw4…@gte.net</u><font =
color=3D"#000000"><br>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~=
~<br><font =
color=3D"#0000FF"><u>http://www.freeyellow.com/members/phoenixresearch/in=
dex.html</u><font =
color=3D"#000000"><br>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~=
~</p>
</font></font></font></font></font></body></html>
——=_NextPart_000_01BC8155.B1FD44E0–

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***Which MICROSCOPE is Pathologists favorite ?***

I am interested in hearing from Pathologists
and Lab Techs doing pathology type microscopy
with comments on their favorite microscopes?

I would be interested in the newer versions
and older scopes and all modalities including
Nomarski and Fluoro.

Your comments as to what constitutes your favorite
scope, and why, would be most appreciated.

Cordially,
Gil Groehn

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Now*accepting <new> and <previously published> writers for publication.
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Reference Searches

ad referendum is the most up to date indexing source on the web.  If you
are looking for up to date references have a look at http://adref.com.
Whereever possible we have linked the references to abstracts or
articles at the site of origin.  I hope you find the site useful

Richard Haig
INfo-Med UK Limited

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Re: !!please !AML, M6(erythremic myelosis or Diguglielmo disease-remission ciretria

- Hide quoted text — Show quoted text -

±èÁ¾¿Ï wrote:

> <AML, M6(erythremic myelosis or Diguglielmo disease-remission ciretria>

> Dear Sir
>   I am a clinical pathologist working for Dankook University Hospital.
>   I would like to have a good opinion for a bone marrow study case.
>   The patient is a 27 year old woman whose bone marrow study shows
>  hypercellular marrow and most of cells composed of erythroid precursor
>  cells, predominently pronormoblasts. Followings are detail.
>   M:E ratio is about 0.043:1(1:22.7).
>   Erythroblasts are increased and counted upto 86.2 % of ANC.
>   Pronormoblasts predominates(about 38.6 % of ANC).
>   Granulopoisis is markedly decreased(5.6%).
>   Nonerythroid blasts are not increased, and they are
>  counted about 0.8% of NEC.
>   Megakarycytes are hardly observed.
>   Dyserythropoietic changes are also observed.
>   In the biopsy section, cellularity is about 95% and marrow space is
>  almostly packed with erythroblasts.
>  So, We diagnosed it as AML, M6(Erythremic myelosis type).
>   After induction chemotherapy, second BM study shows 6% of
>  pronormoblast, 1.5:1 of ME ratio, 20 % of cellularity, and focal
>   erythoid precusors clusters in biopsy specimen.
>  Generally, the focus in the remission criteria of AML is myeloblast
> count.
>  But this bone marrow is richly occupied by erythriod series not
>  associated with a myeloblast component.
>  What I want to know is the remission criteria of erythremic myelosis.
>  Please let me know if you have a good opinion on my case.

>  Sincerely.

>  from JongWan Kim, MD. (wan1…@hitel.kol.co.kr)
>  to sci.med,sci.med.diseases.cancer,
> sci.med.pathology,sci.med.laboratory, sci.med.telemedicine, han.sci.med

A FAB classification purist would probably classify your case as a
myelodysplastic syndrome because of the paucity of type 1 and type 2
myeloblasts although others would as you did classify this case as acute
myeloid leukemia M6B. It is probably a matter of semantics because the
natural history of these cases are similar with a generally poor
response to therapy and short survival and frequent association with
complex karyotypic abnormalities. As far as I know there are no criteria
published specifically for this rare variant of acute myeloid leukemia
but I would consider a patient with this subtype in remission if the
marrow cellularity was greater than 20% with no evidence of dysplastic
pronormoblasts with vacuolated and PAS positive cytoplasm and less than
5% type 1 and type 2 myeloblasts and no detectable Auer rods. In
addition the patient should have an absolute blood neutrophil count of
greater than or equal to 1500/mm3 and a platelet count greater than
100,000/mm3. I would also like to see a normal marrow cytogenetic or
FISH study if the original marrow was abnormal, although the latter is
not considered an absolute criterion for clinical or hematologic
remission.

Richard Miller M.D.
Hematopathology
"alles ist nur ein Uebergang"

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HELP

[

labels.txt < 1K ]

Dear sirs,

we ask you for help or an advice on the following subject.

We are searching for a producer of self-glueing labels good for beeing

printed by a termo-transfer printer. Theese labels will be sticked on

blood-transfusion puches/bags with blood derivates, which are frozen

down to a temperature of minus 40 degrees of Celsius. The need/request

is the maximal resistance and flexibility of the glue, which normally by

such low temperatures breaks, what will cause, the falling of of the

label.

We thank for any information in forward.

With regards, Pavel Stratil

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Prostate pathology

Are there any experts in prostate pathology following this group?
Brad H, MD

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