Does anybody know of a reference source which may help me find this
information?

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- once slides are done, air dry the slides for 30 minutes to 1 hour, then
20 min in chilled acetone (-20C), then store @ -70 until ready to stain

-once the slides are done, immediatley fix in chilled acetone (-20C) for
20 minutes, then air dry for 30 minutes, then store @ -70 until ready to
stain

I will be doing basic H&E’s as well as some immunostaining. I would like
to make sure the tissue is properly fixed as to avoid freezing/fixing
artifacts.

Any advice from people in the pathology field, would be very much
appreciated. Please e-mail your responses as I don’t often get a chance to
read this newsgroup.

Thank you in advance……
—
————-
Marguerite

I’ve had my Liver Function monitored over the last year and
my doctor has drawn a blank as to the causes of elevated
levels of "Gamma Glutayml" and "Alanine Transami".

On the last test (last week) these were 187 (Gamma GT) and
173 (Alanine Transami). All the other liver function tests
are at normal levels (including "Alkaline Phos" which is 69).

For the last 12 months both these tests have been hanging around
these same levels (or 5-10 on either side).

Unfortunately I don’t have any readings prior to this, so I cannot
tell whether they have always been high.

I’ve now had tests for hepatitis (negative) and an ultra-sound of
the liver to spot any  blockages or other problems (there were
none). I have also been totally off alcohol for the last 4-5 months
(I wasn’t a heavy drinker before, but stopped drinking to see if
it made any difference — it didn’t).

I’m not on any medication, I dont smoke or take any other drugs.
My cholestol level is a bit high and I’m currently about 30 pounds
overweight (working on that). I’m male, by the way.

My Doctor has said that he’s pretty much exhausted the possibilities
of what could be causing the two high readings and is putting it
down to my having "naturally high levels" of these readings. He said
I should get readings done every 6 months or so just to make sure
things remain as they are, but he cannot suggest anything else.

My questions….

Firstly, is it possible for people to have high levels of Gamma GT
and Alanine Transami "naturally"?

What other things could these readings be caused by?

Should I be pushing for more investigations, or is my doctor right to
say "dont worry".

Many thanks for any help/suggestions/comments.

Wookie

Does any one have a sensitive procedure for detecting GHB or knows where
I can find one?  GCMS or HPLC.

regards

Michael Torrey
Technical Coordinator
Laboratory Sciences of AZ
mtor…@inficad.com

 http://members.tripod.com/~interhomeless

 http://members.tripod.com/~interhomeless

 Sincerely,
 Valery Kotul

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<Mark1…@mindspring.com> wrote:
>I would be very interested in hearing how others are dealing with the
>impending issue of "Medical Necessity" testing. I am loathe to expend to
>much energy in implementing a specific program directed at these regulations
>until they become less of a moving target, but wonder how rapidly others are
>embracing this concept and what sort of programs are being put into place.
>Mark1…@aol.com

I’ve spent the weekend studying HCFA’s MCP for Clinical Laboratories
and related info.  I understand why there are so many who are playing
the waiting game, but it’s already here. Medicare is, and has been,
refusing payment for tests it doesn’t consider medically necessary,
esp. screening tests (Chem 19s, CBCs, UAs).

AND, get this, HCFA uses the qui tam provisions of the Federal False
Claims Act, aka the whistle-blower provision. This potentially
entitles the people who bring the suits to 15-30% of the final
settlement, so the lawsuits are usually filed by those who work in
your lab or billing dept.

I’m interested in feedback from others who are starting to write
Compliance Plans. I don’t have a lot of help from within the clinic
and would appreciate sharing ideas, etc.

email: ruth…@ovnet.com

Is this the only abnormal LFT?  

One patient with abnormal slightly elevated ALT/AST, GGT was the focus
of a study in this area. The patient had no history of drug/alcohol
abuse; in fact, the only med taken was acetominophen.

But after more thorough hx was taken, we learned that he had been
treated for non-viral hepatitis (from sniffing gasoline fumes) 45
years ago as a small child.

He was advised against any further use of acetominophen and his LFTs
were monitored. It took more than three months, but his nzymes slowly
returned to normal.

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