Pathology and laboratory medicine

pathology, laboratory medicine, clinical pathology laboratories, pathology definition, clinical pathology, pathology laboratory, pathology report, human pathology, lab medicine



I have a question about a procedure I am trying to perfect and was hoping
that you may be somewhat helpful.  I am using 6 micron sections on + slides
and performing a basic immunohistochemical battery (Vector ABC kit).  My
problem involves a high degree of DAB residue (not background) on the
slides.  I have attempted to alleviate this situation by extending washing
periods, I have also tried the procedure on Lysine-subbed slides and am
having the same the residue appear.  The procedure works great on gel
mounted slides but I am unable to use these slides because of a melanin
bleaching procedure required in addition to the traditional
immunohistochemical battery.  Any help you could provide would be greatly


email dkvar…

posted by admin in Uncategorized and have No Comments

Place your comment

You must be logged in to post a comment.