Pathology and laboratory medicine

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help

Hello,

I have a question about a procedure I am trying to perfect and was hoping
that you may be somewhat helpful.  I am using 6 micron sections on + slides
and performing a basic immunohistochemical battery (Vector ABC kit).  My
problem involves a high degree of DAB residue (not background) on the
slides.  I have attempted to alleviate this situation by extending washing
periods, I have also tried the procedure on Lysine-subbed slides and am
having the same the residue appear.  The procedure works great on gel
mounted slides but I am unable to use these slides because of a melanin
bleaching procedure required in addition to the traditional
immunohistochemical battery.  Any help you could provide would be greatly
appreciated.

Sincerely,

David
email dkvar…@rush.edu

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